각종당류가 치아우식원성 세균 mutans streptococci의 대사에 미치는 영향
Effect of sugars on the metabolism of mutans streptococci
김영찬, 정희숙, 김미영, 이행철,
소속 상세정보
김영찬 ( ) - 경희대학교
정희숙 ( ) - 경희대학교
김미영 ( ) - 경희대학교
이행철 ( ) - 경희대학교
KMID : 0355419950190040507
Abstract
Mutans streptococci have been implicated in dental caries. Cariogenicity of the bacteria is attributed to their ability to produce adhesive water-insoluble glucan from sucrose (refined sugar) by which they colonize the teeth. The rationale for
caries
prevention through the use of sugar substitutes or limited use of sugar has been revealed. The effect of sugars, especially xylitol on metabolism of mutans streptococci was investigated. The growth of S. mutans GS5 in brain-heart infusion
broth(BHI)
with 100 mM xylitol was decreased by approx. 50% as compared to that of the bacterium in BHI with glucose, fructose, or sorbitol, as determined by optical density of the growing bacterial cells at 580 nm. The pH drop was least pronounced when S.
mutans
was incubated in BHI containing xylitol. The presence of xylitol affected the pH drop in the BHI containing xylitol. The presence of xylitol affected the pH drop in the BHI containing glucose, suggesting that xylitol can prevent the marked acid
production from glucose by S. mutans. S. mutans GS5, which had been grown in BHI with various sugars and then washed, was incubated with or without 100 mM sucrose in order to observe the ability of the cell-associated glucosyltransferase of the
S.
mutans to produce glucan, and the glucan-mediated binding of to bacterium to smooth surfaces. The washed cells of S. mutans incubated without sucrose failed to produce water-soluble glucan no matter how the organism had been grown with any
sugars.
The
production of water-soluble glucan was most pronounced in S. mutans grown with xylitol. The amount of sucrose in BHI later affected the ability of S. mutans to produce water-insoluble glucan; the bacterium grown in increasing amoounts of sucrose
produced more water-insoluble glucan. The ability of S. mutans to produce water-insoluble glucan was attributed to its binding ability to glass surfaces. Xylitol did not affect the glucan production by and glass-binding of S. mutans. The effect
of
xylitol in binding of 3H-labeled mutans streptococci to hydroxyapatite coated with human saliva (SHA) as a model for the pellicle-coated tooth surface was investigated. Binding of S. mutans GS5 grown in BHI with 100 mM xylitol to SHA was almost
same as
that of the bacterium grown without xylitol. Interestingly, S. mutans NCTC 10449 grown with xylitol, however, showed 3 times greater binding to SHA than the organism grown without xylitol. Overall results indicate that known "noncariogenic" or
"cariostatic" property of xylitol is mainly due to its inhibitory effect on glucose metabolism by mutans streptococci since xylitol affects neither sucrose metabolism by the bacteria with respect to water-insoluble glucan and glucan-mediated
bacterial
colonization to the teeth, nor pellicle-mediated bacterial binding to oral surfaces.
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